TABLE 2.
FISH counts for the white and orange mats, as well the underlying sedimentsa
| Core | Depth (cm) | Total no. of cells (109) | ARC915
|
ANME-2
|
ANME-3
|
EUB I-III
|
DSS658
|
Arc94
|
Mγ705
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of free cells (109) | No. of cells in aggregates (109) | % of total cells | No. of free cells | No. of cells in aggregates (109) | % of DAPI-stained cells | No. of free cells (109) | No. of cells in aggregates | % of total cells | No. of free cells (109) | No. of cells in aggregates (109) | % of total cells | No. of free cells (109) | No. of cells in aggregates (109) | % of total cells | No. of free cells (109) | No. of cells in aggregates | % of total cells | No. of free cells (109) | No. of cells in aggregates | % of total cells | |||
| NL18PC(5) | Mat | 1.00 | 0.03 | < | 3 | < | < | < | < | < | ND | 0.67 | < | 67 | < | < | ND | 0.24 | < | 24 | 0.003 | < | 0.31 |
| (white) | 0-2 | 0.90 | 0.14 | < | 16 | < | < | < | < | < | ND | 0.47 | < | 52 | 0.21 | < | 23 | 0.20 | < | 22 | < | < | ND |
| 2-4 | 2.10 | 0.18 | 0.59 | 37 | < | 0.38 | 18 | < | < | ND | 0.49 | 0.59 | 51 | 0.17 | 0.33 | 23 | 0.10 | < | 5 | < | < | ND | |
| 4-6 | 1.97 | 0.18 | 0.52 | 36 | < | 0.50 | 25 | 0.14 | < | 7 | 0.29 | 0.52 | 42 | 0.07 | 0.23 | 24 | 0.01 | < | 1 | < | < | ND | |
| 6-8 | 0.61 | 0.19 | < | 31 | < | ND | < | 0.17 | < | 27 | 0.15 | < | 25 | 0.03 | < | 5 | < | < | ND | < | < | ND | |
| 8-10 | 1.10 | 0.20 | < | 18 | < | ND | < | 0.07 | < | 6 | 0.41 | < | 37 | < | < | ND | < | < | ND | < | < | ND | |
| 10-12 | 0.42 | 0.07 | < | 18 | < | ND | < | < | < | ND | 0.13 | < | 30 | < | < | ND | < | < | ND | < | < | ND | |
| 12-14 | 0.20 | 0.02 | < | 12 | < | ND | < | < | < | ND | 0.12 | < | 60 | < | < | ND | < | < | ND | < | < | ND | |
| 14-16 | 0.20 | 0.03 | < | 14 | < | ND | < | < | < | ND | 0.02 | < | 11 | < | < | ND | < | < | ND | < | < | ND | |
| 16-18 | 0.24 | ND | ND | ND | < | ND | ND | < | < | ND | ND | < | ND | < | < | ND | < | < | ND | < | < | ND | |
| NL18PC(8) | Mat | 0.80 | 0.02 | < | 3 | < | ND | < | < | < | ND | 0.54 | < | 67 | 0.08 | < | 10 | 0.03 | < | 4 | 0.06 | < | 8 |
| (orange) | 0-2 | 1.28 | 0.03 | 0.07 | 8 | < | 0.10 | 8 | < | < | ND | 0.78 | 0.07 | 66 | 0.17 | 0.07 | 19 | 0.04 | < | ND | 0.03 | < | 2 |
| 2-4 | 4.75 | 0.95 | 0.84 | 38 | < | 0.96 | 20 | < | < | ND | 1.09 | 0.84 | 41 | 0.32 | 1.92 | 7 | 0.03 | < | ND | < | < | ND | |
| 4-6 | 0.76 | 0.19 | < | 25 | < | ND | < | < | < | ND | 0.27 | < | 36 | 0.06 | < | 8 | < | < | ND | < | < | ND | |
| 6-8 | 1.15 | 0.25 | < | 22 | < | ND | < | < | < | ND | 0.30 | < | 26 | 0.14 | < | 12 | < | < | ND | < | < | ND | |
| 8-10 | 1.09 | 0.16 | < | 14 | < | ND | < | 0.08 | < | 7 | 0.38 | < | 35 | 0.03 | < | 3 | < | < | ND | < | < | ND | |
| 10-12 | 0.91 | 0.24 | < | 26 | < | ND | < | 0.06 | < | 7 | 0.16 | < | 17 | 0.09 | < | 10 | < | < | ND | < | < | ND | |
| 12-14 | 1.60 | 0.24 | < | 15 | < | ND | < | < | < | ND | 0.65 | < | 41 | 0.16 | < | 10 | < | < | ND | < | < | ND | |
| 14-16 | 0.97 | 0.15 | < | 16 | < | ND | < | < | < | ND | 0.39 | < | 40 | 0.11 | < | 12 | < | < | ND | < | < | ND | |
| 16-18 | 0.62 | 0.09 | < | 14 | < | ND | < | < | < | ND | 0.15 | < | 24 | ND | < | ND | < | < | ND | < | < | ND | |
a
The number of total cells is the number of cells obtained by AO direct counting. All cell numbers are per ml of sediment. The counts for the ANME-1 and 660 probes were both less than 1% of the total number of cells in all samples. “<” indicates that the number of cells was less than 0.1% of the total number of cells. ND, not determined.