TABLE 3.

Species-specific primers for each poisonous mushroom species

Targeted species	Primer					Product length (bp)
	Name	Length (bp)	Sequence (5′-3′)	Positionb	Tm (°C)c
O. japonicus	OJSP-F	19	GTGCACGTTTCCTTTCAAT	59-77	60.12	107
	OJSP-R	20	AGAATCATCAACAGAGCTGC	165-146	59.55
E. rhodopolius	ERSP-F	22	TTTGAGAACTGCTGTGAAAATC	126-148	60.81	110
	ERSP-R	23	GGCACAAAGTCCCTATATGTTTA	235-213	60.61
C. acromelalga	CASP-F	19	GGTGCACACCTGATAACCA	53-71	62.09	108
	CASP-R	20	AGCTTAAGCTTTCGCACCAG	160-141	63.39
T. ustale	TUSP-F	22	TAGTAGGGACCTCTGTTGCCTT	501-522	62.77	80
	TUSP-R	25	AACCTCCAATTTAAAGCTGCTTCAC	580-556	66.06
Universala	ITS1	19	TCCGTAGGTGAACCTGCGG	Variable	68.4	Variable
	ITS2	20	GCTGCGTTCTTCATCGATGC		68.13
	ITS3	20	GCATCGATGAAGAACGCAGC	Variable	68.13	Variable
	ITS4	20	TCCTCCGCTTATTGATATGC		61.49

^aWhite et al. (12).

^bPositions of nucleotides correspond with sequence of ITS region in each species. The nucleotide sequences were published in the DDBJ nucleotide sequence database under accession numbers AB301601, AB301602, AB301606, and AB036894 respectively.

^cT_m, melting temperature. Values were calculated with oligonucleotide calculator software (Sigma-Aldrich, Tokyo, Japan) on the web site (http://www.genosys.jp/adt/SGJ_NN_3rd.html).