TABLE 1.
Primers and probe for real-time RT-PCR
| Oligonucleotide | Sequencea | Polarity | Locationa |
|---|---|---|---|
| MNV1-RT | 5′-CCATATCCAACTCGAGGTTGGT-3′ | − | 4590-4611 |
| MNV1-FW | 5′-CCTGACATTGTGATGCAAGAATC-3′ | + | 4530-4552 |
| MNV1-probeb | 5′-CGTCATCACCATAGAAG-3′ | − | 4562-4578 |
a
Oligonucleotide sequences and locations were based on the nucleotidic sequence of the MNV CW1 strain (GenBank accession number AY228235).
b
The probe was linked to the fluorophor label 6-carboxyfluorescein and the quencher dye MGBNFQ at the 5′ and 3′ ends, respectively.