FIG. 1.

C. glabrata, C. albicans, and S. cerevisiae LP resistance to H₂O₂. Saturated cultures of C. glabrata (C.g.) strain BG14 and CI MC7 and MC22 (A), C. albicans (C.a.) strain CAI4 and CI CA5 and CA7 (B), and S. cerevisiae (S.c.) strain W303 and CI YJM128 and YJM336 (C) were diluted with fresh medium (YPD) so that all strains reached an OD₆₀₀ of 0.5 after seven doublings at 30°C. C. glabrata and C. albicans strains were divided and exposed to 0, 10, 20, 30, 40, 50, and 100 mM H₂O₂ and S. cerevisiae strains were exposed to 2, 4, 6, and 8 mM H₂O₂ for 3 h. For adaptation experiments, C. glabrata and C. albicans cells were pretreated for 1 h with 10 mM H₂O₂ and then with 100 mM H₂O₂ for 2 additional hours. After the treatment, H₂O₂ was removed by centrifugation. The cultures were resuspended in distilled water, and the OD₆₀₀s were adjusted when needed to 0.5. Cultures were serially diluted, and each dilution was spotted onto YPD plates, ensuring that the same amounts of cells were plated. Plates were incubated at 30°C. See Materials and Methods.