TABLE 3.
Oligonucleotides used in this study
| Primer | Sequencea | Description or restriction site(s) |
|---|---|---|
| 13 | GTTGTAAAACGACGGCCAGTG | pUC forward |
| 17 | GGAAACAGCTATGACCATG | pUC reverse |
| 1 | CGCGGATCCAAGTATACTGCTATGAGCTAC | BamHI |
| 2 | CAAGGAGCTCTTGTGCAGCGTGTAAGATATGAATCAAGTGAT | SacI/BsgI |
| 4 | TCAGATCTCGAGTTGACCGTGACCGAAAC | XhoI |
| 5 | CCGGGTACCTTGTGCAGTCGAAGTTAGAGTGCCCTATTC | KpnI/BsgI |
| 7 | AATCTCGAGTTGTGCAGTGCGGGTAACAATTCTCGGCG | XhoI/BsgI |
| 8 | CCCAAGCTTTTACTTCCTAGTTCTTGTCTC | HindIII |
| 11 | CGCGGATCCTGTCTATATTATCTCGGTAGATC | BamHI |
| 12 | CAAGGAGCTCTTGTGCAGTCAACTCATAGATCACAACATTAACAC | SacI/BsgI |
| 57 | CGCGGATCCTCAATTGTGGGAAGTTATCTAATAAGCC | BamHI |
| 58 | CACTACTAGTTAAACACTTGTAGGAG | SpeI |
| 60 | CCCAAGCTTTTGAACCACGTAAAGTGCTGT | HindIII |
| 61 | CCGGGTACCTTGTGCAGCCTGTAAGGACTTCTAAACC | KpnI/BsgI |
| 2984 | GGTACCCGAGGGAGTTGCCTTCACTATGTGCGT TGAG | KpnI/BcgI |
| 2985 | CTCGAGCTGTTCTTGTTGATCTGTGTTTGGT | XhoI |
| 2986 | GGATTCTGACAGTGTTCCTTATTTTATCTAG | BamHI |
| 2987 | GAGCTCCGACCGCGGTGCATCTGTTACCAGGTTAGCC | SacI/BcgI |
| 2990 | GGTACCCGACTCCTGTGCCTTGTCGTACCAGAGAAAC | KpnI/BcgI |
| 2991 | CTCGAGGAACAGGAATGGACACTAATATATA | XhoI |
| 2992 | GGATCCCCATTCTTTATTTTATTTTCTGCTA | BamHI |
| 2993 | GAGCTCCGAGGCATCTGCTAATAATTTTCCGTTTCAA | SacI/BcgI |
a
Restriction sites are indicated in boldface.