TABLE 2.
Description of oligonucleotide primers used in this work
| Type of expts | Oligonucleotide name | Sequence (5′-3′) | Primer pair target or use |
|---|---|---|---|
| RT-PCR | HM_RT_alpha1_F | CTGTTAACTAAACCGAACAA | α1 of C. glabrata |
| HM_RT_alpha1_R | ATGGGCCATTATATAATTTAC | ||
| HM_RT_a1_F | GTTTACAGATATTGCAATCG | a1 of C. glabrata | |
| HM_RT_a1_R | TGTTTAGATCTTTCGATCTCT | ||
| HM_RT_act1_F | GCTGTCTTGTCTCTATACTCC | ACT1 of C. glabrata | |
| HM_RT_act1_R | CTAGAATAGAACCACCGATC | ||
| HM_RT_a1Scer_R | AAACTCTTACTTGAAGTGGAG | a1 of S. cerevisiae | |
| HM_RT_a1Scer_F | GGATGATATTTGTAGTATGGC | ||
| HM_RT_alpha1Scer_F | CTTCAATATTATTCGACCACT | α1 of S. cerevisiae | |
| HM_RT_alpha1Scer_R | CCAAATGTACAAACACATCT | ||
| HM_YCR097_a1_F | TGTAGTATGGCGGAAAAC | First intron of a1 of S. cerevisiae | |
| HM_YCR097_a1_R | TTGTGTAATGTATGTTGCTC | ||
| HM_YCR097_a1Ter_F | AAGAGAAGAGCCCAAAGG | Second intron of a1 of S. cerevisiae | |
| HM_YCR097_a1Ter_R | ATTTAGATCTCATACGTTTA | ||
| HM_CG_a1_F | GCAATCGGTTAATTCTAATACTC | First intron of a1 of C. glabrata | |
| HM_CG_a1_R | CTGTTTGGGTAAATCTATGTTG | ||
| HM_CG_a1Ter_F | ATCACCACCAATCAAGTTC | Second intron of a1 of C. glabrata | |
| HM_CG_a1Ter_R | TCACGATTGTTTAGATCTTTC | ||
| qRT-PCR | HM_YCR040_alpha1_F | TGTCTTGTCTTCTCTGCTC | α1 of S. cerevisiae |
| HM_YCR040_alpha1_R | ATTATTCGTCAACCACTCTAC | ||
| HM_YCR097_a1Bis_F | AAGAGAAGAGCCCAAAGG | a1 of S. cerevisiae | |
| HM_YCR097_a1Bis_R | CTTGAAGTGGAGTAATGCC | ||
| HM_YCR096_a2_F | CAAAAGAAAATGTCCGAATAC | a2 of S. cerevisiae | |
| HM_YCR096_a2_R | TGAATGCGAGATAAACTGG | ||
| HM_YCR039_alpha2_F | ATTTACCTAAGTTACCAGAGAG | α2 of S. cerevisiae | |
| HM_YCR039_alpha2_R | AGTAGTGAGTTGAGATGTTG | ||
| HM_YFL026_STE2_F | AGCACCATTAACTACACTTC | STE2 of S. cerevisiae | |
| HM_YFL026_STE2_R | CCACATCTGACACCAAAC | ||
| HM_YKL178_STE3_F | ACAAGTCAGCAATAATAGGG | STE3 of S. cerevisiae | |
| HM_YKL178_STE3_R | CCGCATCTACAATACACG | ||
| HM_YDL227_HO_F | GACATTGAAGTTAGAGATTTGG | HO of S. cerevisiae | |
| HM_YDL227_HO_R | ACAGCAGGAGTTACAAGG | ||
| HM_YFL039_ACT1_F | CGCTGCTCAATCTTCTTC | ACT1 of S. cerevisiae | |
| HM_YFL039_ACT1_R | AATCTTTCGTTACCAATAGTG | ||
| HM_CG_alpha1_F | ATTAGAGAAGAAAGGATGAAAC | α1 of C. glabrata | |
| HM_CG_alpha1_R | GATGGAAGTATTGAAGTGATG | ||
| HM_CG_a1Bis_F | AACATATACAACTAGGAAGCC | a1 of C. glabrata | |
| HM_CG_a1Bis_R | TTGAACTTGATTGGTGGTG | ||
| HM_CG_a2_F | GGACCCACAAGAGATAGG | a2 of C. glabrata | |
| HM_CG_a2_R | CGATAGGAAGCGGCATAG | ||
| HM_ CG_alpha2_F | TCAAGAATTAGTATTACGCATC | α2 of C. glabrata | |
| HM_ CG_alpha2_R | AACTGGAACACAATGATATAAG | ||
| HM_CG_STE2_F | TTGCCATTATCCTCTATCTG | STE2 of C. glabrata | |
| HM_CG_STE2_R | ACCCATTGCTCTTATTCG | ||
| HM_CG_STE3_F | GTCTTATGTATTGAACGATTGTG | STE3 of C. glabrata | |
| HM_CG_STE3_R | CCGAGTGCTGATGGAATG | ||
| HM_ CG_HO_F | GCCAAAGAATCATCACAAG | HO of C. glabrata | |
| HM_ CG_HO_R | GCATCCAGATACTCAAGG | ||
| HM_CG_ACT1_F | AGTTGCTGCTTTAGTTATTG | ACT1 of C. glabrata | |
| HM_CG_ACT1_R | CTTGGTGTCTTGGTCTAC | ||
| Strain construction | HM_CgSST2_pUR_F | CGGGATCCTTAAATGATCTGATCCGAGG | Cloning in pUR for construction of |
| HM_CgSST2_pUR_R | GGGGTACCAACTGACTCAATACAGCATC | sst2Δ strains HM103a, HM104a, and HM105a | |
| HM_CgSST2_pRA_F | CGGGATCCTTAGGCGGTTTCTTCATATG | Cloning in pRA for construction of | |
| HM_CgSST2_pRA_R | GGGGTACCAGATTAGACAGAAATCAAGC | sst2Δ strains HM103a, HM104a, and HM105a | |
| HM_CgHMR_pUR_F | CGGGATCCAGGTATGGCTGATGATCAGGA | Cloning in pUR for construction of HMRΔ strain HM102a | |
| HM_CgHMR_pUR_R | GGGGTACCATTTTGATTTTCCGGGACAA | ||
| HM_CgHMR_pRA_F | CGGGATCCGCTTGCAATCAAAGTGTTCTG | Cloning in pRA for construction of HMRΔ strain HM102a | |
| HM_CgHMR_pRA_R | GGGGTACCGCCACTACAAATGAGTTCGTG | ||
| HM-CgURA3_pKA_F | CGGGATCCTTAAAGCTATAGAATCGGCGGGA | Cloning in pKA for construction of ura3Δ strain HM100a | |
| HM-CgURA3_pKA_R | GGGGTACCAGAAATAACTAAGCTCGCGTGGC | ||
| HM-CgURA3_pAN_F | CGGGATCCGTCATTTTCGGAACAATCCGATT | Cloning in pAN for construction of ura3Δ strain HM100a | |
| HM-CgURA3_pAN_R | GGGGTACCTTTGAATTCAGCGAGTTAGCGG |