FIG. 5.

Repressor activity of Efg1p variants in C. albicans. (A) Principle of one-hybrid assay. Basal activity of the lexA operator-ADH1p-lacZ fusion is repressed by the presence of the LexA-Efg1p fusion. (B) Strain CRC106 containing the lexA operator-ADH1p-lacZ fusion was transformed with plasmid Clp-LexA-EFG1 (PC) and its deletion derivatives (D1 to D11); a transformant carrying Clp-LexA was used as a negative control (NC). β-Galactosidase activities of three separate transformants were determined in duplicate assays, and standard deviations were calculated. (C) Plasmid pMC2 carrying expression units for both a LexA-Efg1p fusion protein and a lexA operator-ADH1p-lacZ reporter gene was integrated in the efg1 mutant HLC67 or wild-type strain CAI4. β-Galactosidase activity was determined and compared to the activity of transformants with control plasmid pMC1 lacking the lexA operator sequence. −, pMC1 transformants; +, pMC2 transformants.