. 2008 Mar 14;7(5):791–799. doi: 10.1128/EC.00009-08

TABLE 4.

RT-PCR quantification of the expression level of MIG1

RNA source	C_T^a		Normalized MIG1 level relative to β-actin^b
RNA source	MIG1	β-Actin	Normalized MIG1 level relative to β-actin^b
Wild-type vegetative hyphae	25.82 ± 0.13	21.39 ± 0.07	1.00 (0.91-1.09)
Wild-type infected leaves	26.21 ± 0.22	21.52 ± 0.10	1.39 (1.18-1.65)
mps1 mutant vegetative hyphae	25.88 ± 0.11	21.68 ± 0.04	0.86 (0.79-0.93)

C_T, cycle number at which the fluorescence crossed the threshold. Means and standard deviations were calculated with data from three replicates.

Relative quantification of the MIG1 expression level (23) during plant infection and in the mps1 mutant in comparison to that of vegetative growth. Data are given as the averages (ranges) of three replicates.