TABLE 2.
Ka/Ks ratios for specific regions of ALS1, ALS3, and ALS5
| Region of ALSa | Sequence 1b | Sequence 2b | Ka | Ks | Ka/Ksc | Notes |
|---|---|---|---|---|---|---|
| Ig-like | ALS5 | ALS1 | 0.1084 | 0.3499 | 0.3098 | Somewhat conserved; approx genome avg |
| ALS5 | ALS3 | 0.1295 | 0.4210 | 0.3076 | ||
| ALS1 | ALS3 | 0.1064 | 0.4643 | 0.2291 | ||
| T | ALS5 | ALS1 | 0.0000 | 0.0122 | 0.0000 | Highly conserved |
| ALS5 | ALS3 | 0.0437 | 0.5621 | 0.0777 | ||
| ALS1 | ALS3 | 0.0437 | 0.5368 | 0.0814 | ||
| TR | ALS5 | ALS1 | 0.2278 | 1.3403 | 0.1699 | Conserved |
| ALS5 | ALS3 | 0.2409 | 3.7501 | 0.0642 | ||
| ALS1 | ALS3 | 0.0694 | 1.0566 | 0.0657 | ||
| Stalk | ALS5 | ALS1 | 1.1768 | 1.5999 | 0.7355 | Poorly conserved; many gaps |
| ALS5 | ALS3 | 1.1669 | 1.5981 | 0.7302 | ||
| ALS1 | ALS3 | 0.4685 | 1.0233 | 0.4579 |
The aligned regions were as follows: Ig-like, nucleotides (nt) 327 to 1280; T, nt 1281 to 1586; TR, nt 1587 to 2264; stalk, nt 2265 to 4586 (based on the ALS5 nucleotide sequence).
The acquisition numbers were as follows: ALS5, gi_2522218; ALS1, gi_704426; ALS3, gi_29373982.
Averages were as follows: Ig-like, 0.2822; T, 0.0530; TR, 0.0999; stalk, 0.6412.