TABLE 1.
Strains and plasmids used in this study
| Strain and/or plasmid | Relevant description or genotype | Source or reference |
|---|---|---|
| E. coli K-12 strains and plasmids | ||
| DH5α | supE44 ΔlacU169 (φ80lacZ ΔM15) hsdR17 relA1 recA1 endA1 gyrA96 thi-1 | Laboratory strain |
| MG1655 | F− λ− | Michael Cashel |
| TRMG | MG1655 csrA::kan | 38 |
| TRMG (pPGA372) | cpsE::Tn10; overproduces PGA | 50 |
| XWMGΔC | MG1655 ΔpgaC | 50 |
| TRXWMGΔA | TRMG ΔpgaA | 50 |
| TRXWMGΔB | TRMG ΔpgaB | 50 |
| TRXWMGΔC | TRMG ΔpgaC | 50 |
| TRXWD146 | TRMG pgaD146::cam | 50 |
| pUC19 | Cloning vector, Apr | 40 |
| pCR 2.1-TOPO | Cloning vector, Apr and Kmr | Invitrogen |
| pCRpgaB | pgaB in pCR2.1-TOPO | This study |
| pD115A | pgaB D115A, from pCRPgaB | This study |
| pH184A | pgaB H184A, from pCRPgaB | This study |
| pCRPgaB 271 | pgaB truncation, from pCRPgaB | This study |
| pCRPgaB 410 | Same | This study |
| pCRPgaB 516 | Same | This study |
| Y. pestis strains | ||
| KIM6+ | Hms+ | Joe Hinnebusch |
| KIM6 | Hms− | Joe Hinnebusch |