TABLE 2.

Oligonucleotide primers used in this study^a

Primer name	Sequence 5′ to 3′	Comments
PgaB-SD FWD	GGATCCATAGTGGAGTAATACAGGATGTTACGTAATGGAAATAAATATCTCC	Contains pgaB SD, BamHI for cloning
PgaB REV	TCTAGATTAATCATTTTTCGGATACCAGGC	Contains pgaB stop codon, XbaI for cloning
D115A FWD	GTAGTGCTGACTTTTGATGCCGGCTACCAGAGTTTTTATAC	For site-directed mutagenesis
D115A REV	GTATAAAAACTCTGGTAGCCGGCATCAAAAGTCAGCACTAC	For site-directed mutagenesis
H184A FWD	CTCGTTGAGCTCGCTTCTGCTACATGGAATTCTCACTACGGTATT	For site-directed mutagenesis
H184A REV	AATACCGTAGTGAGAATTCCATGTAGCAGAAGCGAGCTCAACGAG	For site-directed mutagenesis
PgaB-BamHI-SD-FW-2	GGATCCATAGTGGAGTAATACAGG	Contains pgaB SD, BamHI for cloning
PgaB 271	TATTTCTAGATTAGAACATGTCATAACCGAGTTTTTTTAAT	Contains stop codon, XbaI for pgaB deletions
PgaB 410	TATTTCTAGATTAGGGATCTAAATCCCAG	Contains stop codon, XbaI for pgaB deletions
CTTAATACC
PgaB 516	TATTTCTAGATTAGTCAGTTAACGCACGA	Contains stop codon, XbaI for pgaB deletions
CTTTTAAAG

^aAll primers were purchased from Integrated DNA Technologies, Inc., Coralville, IA. Restriction sites are underlined. SD, Shine-Dalgarno.