FIG. 4.
Yeast cells expressing Aga2-GroEL1 on the yeast cell surface adhere to human cells. (A) Untransformed yeast cells or yeast cells expressing Aga2, Aga2-Inv, Aga2-OmcB, or Aga2-GroEL1 from a plasmid were incubated with 1 × 105 HEp-2 cells, and the number of yeast cells associated with HEp-2 cells was determined microscopically. Shown are typical photomicrographs. Bar, 10 μm. (B) Diagrammatic representation of the number of yeast cells expressing Aga2, Aga2-Inv, Aga2-OmcB, and Aga2-GroEL-1 adhering to 1,000 HEp-2 cells (number of experiments = 4). Data shown are means ± standard deviations.