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. 2008 Mar 7;190(10):3489–3493. doi: 10.1128/JB.00146-08

FIG. 4.

FIG. 4.

Holo-CcmE* is not released into the periplasm without CcmD. Periplasmic protein fractions were prepared from E. coli RK103 coexpressing pRGK345 and pRGK362 (lane 1) or pRGK370 and pRGK362 (lane 2). Twenty micrograms of periplasmic proteins was separated by SDS-PAGE and transferred to nitrocellulose. (Top panel) Heme staining showing the levels of holo-CcmE*. (Bottom panel) Immunoblot with CcmE antisera. Abbreviations: Δccm, E. coli RK103; SysIΔE, pRGK345; SysIΔDE, pRGK370; CcmE*:6XHis, pRGK362; αCcmE, CcmE antisera.