TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| A. hydrophila | ||
| SSU | CDCa | |
| SSU-R | Rifr strain of A. hydrophila SSU | Laboratory stock |
| vacB-Ω mutant | Isogenic vacB gene mutant of A. hydrophila SSU-R strain, Rifr Smr Spr | This study |
| vacB-Ω/pBR322 | vacB-Ω mutant strain containing pBR322 vector, Rifr Smr Spr Apr Tcr | This study |
| vacB-Ω/pBR322-vacB | vacB-Ω mutant complemented with the vacB gene via pBR322, Rifr Smr Spr Apr | This study |
| E. coli | ||
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1 deoR araD139 Δ(ara-leu)7697galU galK rpsL (Smr) endA1 nupG | Invitrogen |
| JM109 | endA1recA1gyrA96thihsdR17relA1supE44 λ− Δ(lac-proAB) [F′ traD36proA+B+lacIZΔM15] | Promega |
| SM10 (λpir) | Kmrthi-1thrleutonAlacYsupErecA::RP4-2-Tc::Mu pir | 14 |
| K12 CAN20-12E | RNase I− II− D− BN−, Tcr | 7 |
| K12 CAN20-12ER− | RNase I− II− D− BN− R−, Tcr Kmr | 7 |
| K12 CAN20-12ER−(pBAD) | RNase I− II− D− BN− R−, Tcr Kmr Apr | This study |
| K12 CAN20-12ER−(pBAD/vacB) | RNase I− II− D− BN− R−, Tcr Kmr Apr | This study |
| Plasmids | ||
| pCR2.1 | TA cloning vector, Apr Kmr | Invitrogen |
| pCR2.1/vacB | TA cloning vector carrying the vacB gene, Apr Kmr | This study |
| pHP45Ω | pHP45 plasmid containing a 2-kb Ω element, Apr Smr Spr | 33 |
| pCR2.1/vacB-Ω | TA cloning vector harboring the vacB gene disrupted by Ω element, Apr Smr Spr | This study |
| pJQ200SK | Suicide vector, P15A origin, sacB Gmr | 36 |
| pJQ200SK/vacB-Ω | Suicide vector containing the vacB gene with Ω element, Gmr Smr Spr | This study |
| pBR322 | Apr Tcr | Amersham |
| pBR322/vacB | Contains the vacB gene, Apr | This study |
| pBAD/Thio-E | Arabinose-inducible araBAD promoter-based vector, Apr | Invitrogen |
| pBAD | Control derivative plasmid, Apr | 15 |
| pBAD/vacB | pBAD expression vector with the vacB gene, Apr | This study |
a
CDC, Centers for Disease Control and Prevention, Atlanta, GA.