FIG. 3.
Subcellular locations of PcfC, PcfF, and PcfG. (A) Pcf proteins in total cell (T), cytoplasm (C), and membrane (M) fractions of cCF10-induced CK104(pCF10) (WT) or the mutant strains listed below each panel. Immunoblots were developed with antibodies to the proteins listed at left. (B) Spatial distribution of PcfC, PcfF, PcfG, and PrgB proteins in pheromone-induced CK104(pCF10) cells. Proteins were detected by IFM with antibodies to the proteins listed at the left. Corresponding cells were visualized by Nomarski microscopy (differential interference contrast [DIC]). For each protein, representative fields with multiple cells or magnified individual cells are shown. (C) Corresponding subcellular fractionation (using 20× concentrated fractions) and spatial analyses of PcfC and PcfCΔN103 and PcfCK156T mutant proteins synthesized from the Pnis promoter in the ΔpcfC mutant background.