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Biofilm formation. Strains 168, MM233 (ΔsigB), and MM1722 (ΔsigB amyE::pSpac-yxaB) were analyzed for biofilm formation on synthetic medium at 30°C. (A to C) Overview of the pellicles at 24 to 26 h after inoculation. (D to F) Structure of 24- to 26-h-old biofilm as seen by confocal laser scanning microscopy at a magnification of ×600. Bacteria were visualized using the Live/Dead BacLight kit (Molecular Probes). The IPTG concentration was 1 mM.
