FIG. 4.
Transcriptional activity of the pyxaAB promoter and its derivatives in the presence and absence of AbrB in the bacterial cell. β-Galactosidase activity in strains carrying a lacZ transcriptional fusion to the pyxaAB promoter and its derivatives during planktonic growth in NB medium supplemented with 0.5% glucose with shaking at 37°C is shown. Black bars, 168; open bars, MM1709 (amyE::pyxaAB-lacZ); gray bars, MM1715 (ΔabrB::cat amyE::pyxaAB-lacZ); open bars with horizontal lines, MM1804 (amyE::pyxaAB-4-lacZ); open bars with diagonal lines, MM1808 (ΔabrB::cat amyE::pyxaAB-4-lacZ). The transition state of growth was reached between 4 h and 4.5 h. ONP, o-nitrophenol. Error bars indicate standard deviations.