FIG. 4.
Effect of coaggregation or coculture on S. gordonii growth at low (0.025 mM) arginine concentrations. S. gordonii cells were incubated in monoculture or were mixed with A. naeslundii cells with or without vigorous vortexing to induce the formation of coaggregates. Growth of S. gordonii DL1 (A) in monoculture (•), coculture without vortexing (▪), or coaggregates (▴) or growth of S. gordonii PK3337 (B) in monoculture (•) or coaggregates (▴). (C to H) Phase-contrast micrographs of S. gordonii DL1 coaggregates (C, E, and G) and cocultures (D, F, and H) with A. naeslundii after 0 h (C and D), 10 h (E and F), or 24 h (G and H). Large coaggregates are indicated by arrows. Bar, 20 μM.