TABLE 1.
Peptidoglycan nucleotide precursor pools in cells from strain 168 and strain BFS2823 with or without IPTG
| Strain | Pool level (pmol/mg of cell protein)a
|
|||
|---|---|---|---|---|
| UDP-MurNAc pentapeptide | UDP-MurNAc tripeptide | UDP-MurNAc dipeptide | UDP- GlcNAc | |
| 168 | 90 | 3 | 8 | 162 |
| BFS2823 (without IPTG) | 530 | 23 | 114 | 187 |
| BFS2823 (with IPTG) | 60 | 5 | ND | 196 |
a
The peptidoglycan precursor fraction was obtained from a 2-liter exponential culture in E-medium as previously described (3). After a gel filtration step (Sephadex G-25); excluded fractions absorbing at 262 nm were pooled, lyophilized, and analyzed by HPLC as described previously (29, 30). Values are the average of two measurements. ND, not determined.