TABLE 1.
Strain | Pool level (pmol/mg of cell protein)a
|
|||
---|---|---|---|---|
UDP-MurNAc pentapeptide | UDP-MurNAc tripeptide | UDP-MurNAc dipeptide | UDP- GlcNAc | |
168 | 90 | 3 | 8 | 162 |
BFS2823 (without IPTG) | 530 | 23 | 114 | 187 |
BFS2823 (with IPTG) | 60 | 5 | ND | 196 |
The peptidoglycan precursor fraction was obtained from a 2-liter exponential culture in E-medium as previously described (3). After a gel filtration step (Sephadex G-25); excluded fractions absorbing at 262 nm were pooled, lyophilized, and analyzed by HPLC as described previously (29, 30). Values are the average of two measurements. ND, not determined.