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. 2008 Mar 14;190(10):3681–3689. doi: 10.1128/JB.00846-07

FIG. 2.

FIG. 2.

Analysis of the region in pSW100 that affects plasmid stability. E. coli HB101(pSW100K), E. coli HB101(pSW103K), E. coli HB101(pSW116K), E. coli HB101(pSW117K), E. coli HB101(pSW119K), E. coli HB101(pSW130K), and E. coli HB101(pSW142K) were cultured in LB-Km broth overnight and used to inoculate LB broth. Cells were subcultured every 12 h (seven generations), plated on LB agar, and then replica plated on LB-Km agar to determine the numbers of the colonies that did not contain the plasmid.