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. 2008 Mar 14;190(10):3681–3689. doi: 10.1128/JB.00846-07

FIG. 6.

FIG. 6.

Intracellular localization of pSW128A and TraC. E. coli BL21(DE3)(pSW128A, pML13) (A) or E. coli BL21(DE3)(pSW129A, pML13) (B) was cultured at 37°C overnight. (a) TraC protein stained by indirect immunofluorescence staining using anti-His polyclonal antibody and Alexa Fluor 488-conjugated anti-rabbit secondary antibody (green); (b) plasmid DNA visualized by FISH using an Alexa Fluor 546 ULS (Universal Linkage System)-labeled probe (red); (c) chromosome stained by DAPI (blue); (d) merged image.