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Southern blot analysis of traC in E. coli and P. stewartii subsp. stewartii. (A) Five micrograms of DNA from E. coli DH5α (lane 1) and E. coli HB101 (lane 2) was digested with BglII/NotI. Lane 3 was loaded with P. stewartii subsp. stewartii SW2 plasmids that had been purified by an alkaline lysis method (26). (B) Southern hybridization was performed using a biotin-labeled traC probe.
