TABLE 1.
Relevant bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| CFT073 | Wild-type pyelonephritis isolate (O6:K2:H1) | 45 |
| CFT073 fim L-ON | CFT073 fim IE phase locked ON; constitutive type 1 fimbrial expression | 27 |
| CFT073 fim L-ON Nalr | Nalr derivative of CFT073 fim L-ON | This study |
| CFT073 Δlrp | CFT073 Δlrp::kan; replacement of nucleotides 41 to 428 of lrp in CFT073 with the kanamycin resistance gene of pKD4 | This study |
| CFT073 ΔlrhA | CFT073 ΔlrhA::kan; replacement of nucleotides 41 to 889 of lrhA in CFT073 with the kanamycin resistance gene of pKD4 | This study |
| CFT073 ΔmutS | CFT073 ΔmutS::kan; replacement of nucleotides 99 to 2332 of mutS in CFT073 with the kanamycin resistance gene of pKD4 | This study |
| CFT073 ΔydiV | CFT073 ΔydiV::kan; replacement of nucleotides 91 to 552 of ydiV in CFT073 with the kanamycin resistance gene of pKD4 | This study |
| CFT073 ΔslyA | CFT073 ΔslyA::kan; replacement of nucleotides 46 to 345 of slyA in CFT073 with the kanamycin resistance gene of pKD4 | This study |
| CFT073 ΔpapX | CFT073 ΔpapX::kan; replacement of nucleotides 1 to 550 of papX in CFT073 with the kanamycin resistance gene of pKD4 | This study |
| CFT073 fim L-ON Δlrp | CFT073 fim L-ON Δlrp::kan; replacement of lrp (as in CFT073 Δlrp) in CFT073 fim L-ON | This study |
| CFT073 fim L-ON ΔlrhA | CFT073 fim L-ON ΔlrhA::kan; replacement of lrhA (as in CFT073 ΔlrhA) in CFT073 fim L-ON | This study |
| CFT073 fim L-ON ΔmutS | CFT073 fim L-ON ΔmutS::kan; replacement of mutS (as in CFT073 ΔmutS) in CFT073 fim L-ON | This study |
| CFT073 fim L-ON ΔydiV | CFT073 fim L-ON ΔydiV::kan; replacement of ydiV (as in CFT073 ΔydiV) in CFT073 fim L-ON | This study |
| CFT073 fim L-ON ΔslyA | CFT073 fim L-ON ΔslyA::kan; replacement of slyA (as in CFT073 ΔslyA) in CFT073 fim L-ON | This study |
| CFT073 fim L-ON ΔpapX | CFT073 fim L-ON ΔpapX::kan; replacement of papX, as in CFT073 ΔpapX, in CFT073 fim L-ON | This study |
| Plasmids | ||
| pUT/mini-Tn5Km2 | Mini-Tn5 Km2 transposon in pUT suicide vector (Kanr Ampr) | 18 |
| pKD4 | Lambda red recombinase system template plasmid (Kanr Ampr) | 17 |
| pGEN-MCS | Derivative of pGEN222 with multiple cloning site replacing gfpuv (Ampr) | 33 |
| pGEN-lrp | lrp (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr) | This study |
| pGEN-lrhA | lrhA (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr) | This study |
| pGEN-mutS | mutS (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr) | This study |
| pGEN-ydiV | ydiV (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr) | This study |
| pGEN-slyA | slyA (with native promoter) of CFT073 cloned into BamHI-SphI sites of pGEN-MCS (Ampr) | This study |
| pLX3607 | pQE60 cloning vector with lacIq cloned into XhoI site (Ampr) | 37 |
| pDRM001 | papX of CFT073 cloned into NcoI-HindIII sites of pLX3607 (Ampr) | 37 |