TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Genotype and/or relevant informationa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | supE44 lacU169 (80lacZM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 8 |
| S. pneumoniae | ||
| GTC13809 | Wild-type encapsulated (serotype 19) | Gifu University |
| HT1 | spxB::Kanr derivative of GTC13809 | This study |
| HT2 | lox::Spcr derivative of GTC13809 | This study |
| Plasmids | ||
| pGEM-T Easy | Apr | Promega |
| pBRΩKm-2 | Kmr | 10 |
| pUC19 | Apr | Stratagene |
| pSET4s | lacZ′, Spr | 32 |
| pTN101 | pUC19 carrying lox fragment | This study |
| pTN102 | pGEM-T Easy carrying Spr cassette | This study |
| pTN103 | pTN101 carrying lox::Spr | This study |
| pTN201 | pGEM-T Easy carrying spxB fragment | This study |
| pTN202 | pTN201 carrying spxB::Kmr | This study |
a
Apr, ampicillin resistance; Spcr and Spr, spectinomycin resistance; Kanr and Kmr, kanamycin resistance.