TABLE 2.
PCR primers used
| Primer | Sequence (5′-3′)a | Descriptionb |
|---|---|---|
| Spx1 | GGAATTCCATAATCTTTTAGGAGTGGTTCG | Forward primer for spxB |
| Spx2 | CGGGATCCCGCAACAGCTCTTTGAGCTTCC | Reverse primer for spxB |
| Lox1 | CGGAATTCCGGCAGCATTTGGCTATATCGC | Forward primer for lox |
| Lox2 | CCCAAGCTTGGGATGGATTGTGACGGAGCTTG | Reverse primer for lox |
| Sp1 | ATGCATGCATGTTCGTGAATACATGTTATA | Forward primer for Spr cassette |
| Sp2 | ATGCATGCATGTTTTCTAAAATCTGAT | Reverse primer for Spr cassette |
| Spx-pr1 | GTTGTCGGAATTCCGATTGC | Forward primer for spxB probe |
| Spx-pr2 | CTTTGTCTTCAGCCAAAGCG | Reverse primer for spxB probe |
| Lox-pr1 | CGGAATTCCGGCAGCATTTGGCTATATCGC | Forward primer for lox probe |
| Lox-pr2 | GCGCAGTCGCCACTTCCCCC | Reverse primer for lox probe |
a
Underlined are restriction site adapters. Lox-pr1is identical to Lox1.
b
These primers were designed on the basis of the genome sequence of strain TIGR4 (accession no. NC_003028).