FIG. 5.

Verification of the newly predicted genes as part of the AphB regulon. Fourteen genes from the pool of AphB regulon members newly predicted on the basis of microarray analysis results were analyzed by RT-PCR. Each column represents the mRNA expression level in the aphB mutant relative to that in the wild type. Averages and SEM were calculated from the results of at least three independent experiments. Locus tags are based on the database of the V. vulnificus CMCP6 genome as described in the legend to Fig. 4, and the products of the 14 genes are listed on the right. PTS system, phototransferase system.