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Gas chromatograms of the alditol acetate derivatives from serotype 4 (A) and serotype 16 (B) GPLs. Total ion chromatograms (TIC) are shown. GC was performed on an SPB-1-fused silica column with a temperature program of 160°C for 2 min, followed by an increase of 4°C/min to 220°C, and holding at 220°C for 15 min. Comparison to the GC spectrum of serotype 4 GPL shows that serotype 16 GPL is composed of 3,4-di-O-Me-Rha, 4-O-Me-Rha, Rha, 6-d-Tal, and an unknown X1 sugar residue.
