FIG. 2.

Opr86 plays a role in OMP assembly. (A, B) Examination of IMP (A) and OMP (B) levels by SDS-PAGE stained with Coomassie brilliant blue. IMPs and OMPs were extracted from homogenized cells at the 9-h growth point prior to the dilution described in the legend for Fig. 1B. WT, PAO1 wild type; PS186 +IPTG, PS186 cultured with IPTG; PS186 −IPTG, PS186 cultured without IPTG. (C) Protein levels from four fractions of OMPs from PS186 cultured with and without IPTG were quantified and expressed as ratios relative to levels of PAO1 OMPs and SD from three independent experiments.