FIG. 7.

Immunological biofilm inhibition is effective against clinical strains. (A) Immunological influences on biofilm formation by purified IgG. PAO1 and four different clinical strains were preincubated with anti-PAO1, anti-o-Opr86, non-immunized IgG (NC IgG), or PBS and inoculated into microtiter wells containing 100 μl of LB medium. The IgG concentration in the medium was 400 μg/ml. Biofilms were allowed to form for 8 h. Biofilms attached to the wells were stained with crystal violet, solubilized in ethanol, and measured at OD₅₉₅. Data shown are representative of three independent experiments. The results are expressed as the means and SD from at least four wells per sample. (B) The amino acid sequences of the o-Opr86 region. The sequence from amino acids 651 to 700 of opr86 in PAO1 and sequences of corresponding regions of clinical strains 401, 428, 443, and 287 are shown. Amino acids different from those of PAO1 are boxed in black.