TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| Strain | ||
| P. aeruginosa | ||
| PAO1 | Wild type (formally PAO1-Tokai) | 29 |
| PAO1-H | Wild type (formally PAO1-Holloway) | 15 |
| PS186 | PAO1 opr86 mutant carrying pMMB6786 | This study |
| 287 | Clinical isolate | Okayama University |
| 401 | Clinical isolate | Okayama University |
| 428 | Clinical isolate | Okayama University |
| 443 | Clinical isolate | Okayama University |
| E. coli | ||
| JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+ lacIqlacZΔM15] | TaKaRa |
| BL21 | E. coli B, F−dcm ompT hsdS(rB+ mB+) gal | TaKaRa |
| S17-1 | pro thi hsdR recA Tpr Smr; chromosome::RP4-2 Tc::Mu-Km::Tn7 | 45 |
| Plasmid | ||
| pMMB67EH | Broad-host-range vector; Ampr IncQ | 10 |
| pMMB6786 | pMMB67EH derivative carrying the opr86 gene | This study |
| pHSG398 | Cloning vector; Cmr | TaKaRa |
| pG19II | Derivative of pK19 mob sacB; Gmr | 30 |
| pGEX6P1 | Cloning vector; Ampr | GE Healthcare |
| pGEX6p86 | pGEX6P1 derivative carrying p-opr86 | This study |
| pGEX6o86 | pGEX6P1 derivative carrying o-opr86 | This study |