TABLE 2.
Susceptibilities to various detergents and antibiotics
| Compounda | Zone of growth inhibition (mm)b
|
||
|---|---|---|---|
| PAO1 | PS186 (IPTG 100 μM) | PS186 (IPTG 10 μM) | |
| 10% SDS | 0 | 0 | 0 |
| 5% CTAB | 4.0 (±0) | 4.3 (±0.4) | 4.3 (±0.4) |
| 10% Tween 20 | 0 | 0 | 4.7 (±0.5) |
| 10% Triton X-100 | 0 | 0 | 3.7 (±0.5) |
| 0.5 M EDTA | 6.7 (±0.5) | 7.0 (±0.8) | 6.7 (±0.5) |
| 1% Polymyxin B | 11.3 (±0.5) | 11.3 (±0.5) | 12.6 (±0.5) |
| 1% Nalidixic acid | 13.3 (±0.9) | 14.0 (±0.8) | 15.7 (±1.2) |
| 1% Ofloxacin | 18.0 (±0.8) | 17.6 (±0.5) | 26.7 (±1.7) |
| 1% Rifampin | 7.3 (±0.9) | 7.3 (±0.5) | 10.6 (±0.9) |
| 1% Erythromycin | 5.0 (±0) | 5.0 (±0) | 8.0 (±1.4) |
| 1% Chloramphenicol | 13.0 (±0) | 14.0 (±0.8) | 19.0 (±1.6) |
| 1% Tetracycline | 15.0 (±1.6) | 17.3 (±1.7) | 19.3 (±1.7) |
| 1% Gentamicin | 11.0 (±0) | 11.3 (±0.5) | 12.3 (±0.5) |
a
The compounds were spotted (40 μl) in a cup placed on M63 minimal medium (supplemented with glucose and Casamino Acids as carbon sources) agar containing a lawn of cells plated at 106 cells/ml. Nalidixic acid, ofloxacin, and rifampin were resuspended in 0.02 N NaOH, 0.01 N HCl, and 50% methanol, respectively. Erythromycin, chloramphenicol, and tetracycline were resuspended in 50% ethanol. The zones were measured after 12 h at 37°C. CTAB, cetyltrimethylammonium bromide.
b
The values are expressed as the averages for triplicate assays. The numbers in parentheses indicate SD.