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Differential inhibition of the PDH complex from wild-type E. coli strain W3110 (Native) and the two LPD mutant derivatives by NADH. The NAD+ concentration (0.5 mM) was at least five times the Km value. The specific activity of the native enzyme complex without NADH was 40.16 U. The specific activities of the two PDH complexes with LPD mutations H322Y and E354K without NADH were 17.74 and 31.00 U, respectively.
