TABLE 1.
Bacterial strains, plasmids, and phages used in this study
| Strain, plasmid, or phage | Relevant genotype | Source or reference |
|---|---|---|
| E. coli K-12 strains | ||
| W3110 | Wild type | ATCC |
| AH241 | W3110, ΔldhA | 19 |
| AH242 | W3110, ΔldhA Δ(focA-pflB)-Km | 19 |
| BW25113 | lacIqrrnBT14 ΔlacZW116hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | B. Wanner |
| JM109(λDE3) | F′ [traD36 proA+B+ lacIq Δ(lacZ)M15] Δ(lac-proAB) glnV44 e14−gyrA96 recA1 relA1 endA1 thi hsdR17 (λDE3) | Promega |
| SE2377 | AH242, lpd102(H322Y) | This study |
| SE2378 | AH242, lpd101(E354K) | 19 |
| SE2382 | AH242, lpd101(E354K) | This study |
| SE2383 | AH242, lpd102(H322Y) | This study |
| SE2384 | AH242, lpd102(H322Y) | This study |
| SE2385 | AH242, lpd101(E354K) | This study |
| YK1 | SE2378, Kms | This study |
| YK29 | AH242, Kms | This study |
| YK87 | YK1, ΔadhE-Km | This study |
| YK100 | AH242, Δlpd | This study |
| YK110 | YK100, lpd+ | YK100 × P1(W3110) |
| YK111 | YK100, lpd101 | YK100 × P1(SE2378) |
| YK128 | YK100, Plpd+ (pKY32) | This study |
| YK129 | YK100, Plpd101 (pKY33) | This study |
| YK139 | YK100, lpd102 | YK100 × P1(SE2377) |
| YK141 | YK100, lpd101 | YK100 × P1(SE2382) |
| YK175 | AH241, ΔadhE-Km | AH241 × P1(YK87) |
| YK176 | YK141, ΔadhE-Km | YK141 × P1(YK87) |
| YK181 | YK139, ΔadhE-Km | YK139 × P1(YK87) |
| Plasmids | ||
| pKY10 | pUC19, PpdhRSE2378 | This study |
| pKY13 | pUC19, PpdhR+ | This study |
| pKY15 | pTL61t, PpdhSE2378-lacZ | This study |
| pKY17 | pTL61t, PpdhW3110-lacZ | This study |
| pKY32 | pTrc99a, lpd+bla | This study |
| pKY33 | pTrc99a, lpd101 bla | This study |
| pKY36 | pET15b, lpd+bla | This study |
| pKY37 | pET15b, lpd101 bla | This study |
| pKY38 | pET15b, lpd102 bla | This study |
| Phages | ||
| λYK1 | λ(PpdhW3110-lacZ) | This study |
| λYK2 | λ(PpdhSE2378-lacZ) | This study |