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. 2008 Apr 4;190(11):3940–3947. doi: 10.1128/JB.00086-08

FIG. 4.

FIG. 4.

TonB-dependent cell association of 55Fe and [3H]norepinephrine. (A) B. bronchiseptica strains B013N (tonB+), BRM31(pBBR1MCS- 1) (tonB::pSSt2), and BRM31(pBB41) (tonB::pSSt2/tonB+) were used. Bacteria were grown in iron-depleted medium containing 200 μg/ml 55Fe-transferrin (solid bars), 200 μg/ml 55Fe-transferrin plus 50 μM norepinephrine (shaded bars), or 200 μg/ml 55Fe-transferrin plus 50 μM norepinephrine plus 36 μM FeSO4 (open bars). (B) B. bronchiseptica strains B013N (solid bars), BRM31(pBBR1MCS-1) (shaded bars), and BRM31(pBB41) (open bars) were used. Iron-depleted basal medium containing [3H]norepinephrine was supplemented with either 200 μg/ml transferrin plus 50 μM norepinephrine (FeTf + NE) or 200 μM transferrin plus 50 μg/ml norepinephrine plus 36 μM FeSO4 (FeTF + NE + FeSO4). Cell-associated levels of 55Fe or [3H]norepinephrine were determined by scintillation counting and normalized based on the optical density at 600 nm (OD600) after 24 h of incubation. Values represent means ± 1 standard deviation (error bars) from triplicate determinations.