TABLE 1.
B. bronchiseptica TonB-dependent receptor genes
| Locus tag | Gene name and/or reference | Primer sequencea (5′→3′) |
|---|---|---|
| BB0078 | bfrC (6) | GGCCGAATTCGCCGGTGCGCAACGACAATGAC |
| GGCCAAGCTTCGCGGCCGCCACGAACT | ||
| BB0189 | This study | GCGCGGATCCTGCCCGGCCACAACCACGAGT |
| CGCGGAATTCTGCGGCGCGAAGTCCCAGATG | ||
| BB0832 | 41 | GCGCGGATCCCGTAGCGCCGGCCGAACAGGT |
| GCGCGAATTCCGCCGCGGCTACGACAACTACA | ||
| BB1294 | bfrG (41) | GGCCGAATTCGCCGACCGCGATTTCCAGA |
| GGCCAAGCTTCGGCGCGGCGTGTTGACC | ||
| BB1785 | bfrI (41) | GGCCGAATTCCGGCGGCGTGGTCAACCTCGTCAG |
| GGCCAAGCTTGGTGGCGTTGTCGGTTTCCTTGTC | ||
| BB1846 | bfrB (6) | GGCCGAATTCGTATTTCATCCGCGGCTTCATCCT |
| GGCCAAGCTTCGGCGGCGGGCGAGTTGTGG | ||
| BB1905 | 41 | CCGGGGATCCGCCGCGCTCGTCCGTCTATGTGTC |
| GATCCGTGGCGTCCGTGAACTG | ||
| BB1942 | bfeA (8) | NAb |
| BB2179 | This study | CGCGGAATTCACACATAATCGGCCCGTTCCACA |
| GCGCGGATCCCCCGGTCAGCCATGCCTCCTA | ||
| BB3658 | bfrH (41) | GGCCGAATTCCCGGCGGGTCAACATGGATTTTTC |
| GGCCAAGCTTCCCGGCGGCTGGTATTTCACG | ||
| BB3825 | bfrE (41) | GGCCAAGCTTGCGGCGGGCAGCATCTTCGTC |
| GGCCAAGCTTTTGTCTTCGCGGCTGGTGCTGTAG | ||
| BB3826 | bfrD (42) | GGCCGAATTCGTCAGCCGCCGCAACTTCTACG |
| GGCCAAGCTTCGGCGACTTCGATGCTGGTGTT | ||
| BB3900 | fauA (10) | NA |
| BB4122 | bfrF (41) | GGCCGAATTCCAGCGCGATGTCCGAACCCTACG |
| GGCCAAGCTTGCGGCCGCTGAGCACCACCAC | ||
| BB4457 | This study | CGCGGAATTCCTCAACGGCCAGCGCATCAACAA |
| GCGCGGATCCGCCGGTGGGGGTGCTCATCTG | ||
| BB4655 | bhuR (55) | NA |
| BB4744 | bfrZ (44) | GCGCGAATTCCGGGCGGCATCGTCAACTACACCT |
| CTTGCTGCGGCCCTGCGAGAACG | ||
| BB4761 | bfrA (7) | GCGCGAATTCGCGCGGCCCTATGTCCACCCTG |
| GCGCGGATCCCTCCACTTGCCGCCGAACTGCTC | ||
| BB4956 | hemC (41) | GCGCGAATTCGGCACCCGCTTCGCATACTCG |
| GCGCGGATCCCGCGCCGCCTTCCAGACCAC |
a
Primer sequences for PCR amplification of internal gene fragments used in the construction of insertion mutations.
b
NA, not applicable.