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Purification and autophosphorylation of MBP fusion proteins MBP-NarX185 (MX) and MBP-NarQ182 (MQ). (A) The purification of MBP fusion proteins was analyzed on a 10% Laemmli gel stained with Coomasie Blue (GelCode Blue Stain Reagent; Pierce) by FluorChem. The relative positions of molecular weight references are indicated. The amount of protein applied for each lane was 1.6 μg. (B) Radiolabeled proteins were prepared and visualized as stated in Materials and Methods.
