TABLE 1.

Kinetic values of autophosphorylation and dephosphorylation reactions

Protein	Kinetic valueb				Reference
	Autophosphorylation		Dephosphorylation
	KmATP (μM)	k (10−4) (s−1)	KmADP (μM)	−k (s−1)
MBP-NarX185a	2.4 ± 0.7	0.5 ± 0.2	2.3 ± 0.6	0.029 ± 0.01	This study
MBP-NarQ182a	22.8 ± 9.3	2.2 ± 1.1	1.8 ± 0.7	0.013 ± 0.003	This study
CheA	300 ± 75	260 ± 40	42 ± 8	0.028 ± 0.003	35
NRII (NtrB)	31 ± 1.4	118 ± 2.5	40	−	16, 34
KinA	74 ± 11	19 ± 5	16 ± 2.6	−	15
EnvZ	218	0.81c	−	−	17
PhoQ	20.1 ± 13.1	−d	−	−	21

^aValues were determined as described under Materials and Methods and Results expressed per monomer.

^b± standard errors of the mean. For the Nar sensors, these values were determined from three independent measurements and different protein preparations.

^cEstimated rate taken from Fig. 3 (17).

^d−, not determined or published.