Structural organization of FnBPA from S. aureus 8325-4 and diagrammatic illustration of plasmid constructs lacking regions of FnBPA. Regions B, C, and D (tandem repeats 1 to 11) are required for fibronectin binding. Region A (comprising the subdomains N1, N2, and N3) binds fibrinogen and elastin. The LPXTG motif, required for sortase-mediated anchoring of the protein to the cell wall peptidoglycan, is indicated. The secretory signal sequence (S), wall-spanning (W), and membrane-spanning (M) domains are indicated.