FIG. 5.

T4P-mediated chemotaxis of P. aeruginosa strains up a gradient of either PE or LCFA (palmitoleic acid, C₁₆) in the presence of 0.5% glucose that was incorporated throughout the agar. In all cases, the PE or palmitoleic acid gradient was first established to the right of the round/oval ring marking where the culture of P. aeruginosa was placed on the top of the agar 24 h later (see Materials and Methods). (A) PAO1 ΔaceA mutant with palmitoleic acid as the chemoattractant; (B) PAO1 wild type with palmitoleic acid as the chemoattractant; (C) PAO1 ΔaceA mutant with PE as the chemoattractant; (D) PAO1 wild type with PE as the chemoattractant.