FIG. 2.

Northern blot of xcinA and ximB genes. Total RNA was obtained from uninduced and mitomycin C-induced X. nematophila cells at different times. Ten micrograms of RNA was used in each lane. (A) Blot probed with labeled xcinA gene. Lanes 1 to 4, RNA from uninduced cells at 0, 1, 2, and 3 h, respectively, with ethidium bromide-stained bands corresponding to the 16S RNA of the gel as a loading control; lanes 5 to 8, RNA from mitomycin C-induced cells at 0, 1, 2, and 3 h, respectively; lane M, 3-kb RNA marker. (B) Blot probed with labeled xcimB gene. Lanes 1 to 4, RNA from uninduced cells at 0, 1, 2, and 3 h, respectively; lanes 5 to 8, RNA from mitomycin C-induced cells at 0, 1, 2, and 3 h, respectively, with ethidium bromide-stained bands corresponding to 16S RNA in the gel used as a loading control.