FIG. 6.

RNase activity of the purified catalytic domain. Each 20-μl reaction mixture containing 1.2 μg of RNA with the test protein was incubated at 37°C for 1.5 h. Lane 1, RNA without protein; lane 2, RNA with 10 μg of catalytic domain-immunity domain complex; lane 3, 10 μg heat-inactivated catalytic domain; lanes 4, 5, and 6, RNA with 10, 5, and 1 μg catalytic domain, respectively; lanes 7 and 8, 5 μg catalytic domain-immunity domain protein at molar ratios of 1:3 and 1: 1, respectively; lane 9, 5 μg catalytic domain-bovine serum albumin at a molar ratio of 1:2; lane 10, E. coli DNA without protein; lane 11, DNA with 5 μg catalytic domain protein.