TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid/strain | Characteristics | Source |
|---|---|---|
| E. coli strains | ||
| DH5α | supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 φ80dlacZΔM15 | Invitrogen |
| M15 | NaIs Strs Rifs Thi− Lac− Ara+ Gal+ MtI− F− RecA+ Uvr+ Lon+ | Qiagen |
| BL21(DE3)/pLysS | F−ompT hsdSB(rB− mB−) gal dcm(DE3)/pLysS(Cmr) | Novagen |
| Plasmids | ||
| pBSK(+) | 2.9-kb cloning vector; Ampr | Stratagene |
| pGEM-T Easy | 3-kb vector for cloning PCR fragments; Ampr | Promega |
| pQE30 | 3.4-kb expression vector; Ampr | Qiagen |
| pET28 | 5.3-kb expression vector; Kanr | Novagen |
| pBSK4.3/BSK4.3 | pBSK(+) containing 4.3-kb insert from X. nematophila genome | This study |
| pGEM1/GEM1 | pGEM-T Easy containing xcinA and ximB genes with native promoter | This study |
| pBSK1/BSK1 | pBSK(+) containing xcinA and ximB genes with native promoter | This study |
| pGEM2/GEM2 | pGEM-T Easy containing xcinA alone with native promoter | This study |
| pBSK2/BSK2 | pBSK(+) containing xcinA alone with native promoter | This study |
| pJC1/JC1 | pGEM-T Easy containing xcinA and ximB genes | This study |
| pJC2/JC2 | pQE30 containing xcinA amd ximB genes | This study |
| pJC3/JC3 | pET28 containing 318-bp catalytic domain | This study |
| pJC4/JC4 | pET28 containing catalytic domain and 270-bp partial ximB (N′-terminal 86 amino acids) | This study |
| pLacZ/LacZ | pGEM-T Easy containing lacZ coding sequence | This study |
| pXcinPr/XcinPr | pGEM-T Easy containing 300-bp upstream sequence of xcinA | This study |
| pXPro-lacZ/XPro-lacZ | pGEM-T Easy containing xcinA upstream sequence fused to lacZ coding sequence | This study |
| pXimPr/XimPr | pGEM-T Easy containing 300-bp upstream sequence of ximB | This study |
| pIPro-lacZ/IPro-lacZ | pGEM-T Easy containing ximB upstream sequence fused to lacZ coding sequence | This study |