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. 2008 Mar 28;190(11):4017–4026. doi: 10.1128/JB.00117-08

FIG. 4.

FIG. 4.

The TSS of mtaA1 and mtbA in M. acetivorans determined by 5′ RLM-RACE. The 5′ mRNA leader region upstream of the putative start codon (cyan and underlined bases) for the three genes along with the experimentally determined TSS(arrow) and the putative TATA box (black bracket) and RBS (orange text) are shown. Panel A shows this region for the mtaA1 promoter; the red letters represent bases conserved in all three Methanosarcina spp., namely M. acetivorans (Ma), M. mazei (Mm), and M. barkeri (Mb). Panel B shows the 5′ mRNA leader region for the mtbA promoter; the blue letters represent bases conserved in all three Methanosarcina spp. while the red letters represent bases conserved between M. acetivorans and M. mazei.