TABLE 2.
Primers used in the study
| Primer | Sequencea | Added site(s) |
|---|---|---|
| 5-upmtaA | GGGGGGAAGCTTCTCGAGAGCCAGGATGTCCTTCACC | HindIII, XhoI |
| 3-upmtaA | GGGGGGGGTACCCATATGGGTCATACC | KpnI, NdeI |
| 5-dnmtaA | GGGGGGGGTACCAGATCTTGAAAACGCATAAAAACCC | KpnI, BglII |
| 3-dnmtaA | GGGGGGTTCGAACCTAGGTAACAGCGCAGGTTCTCTCC | BstBI, AvrII |
| SpeI-upA2 | GCCGCCACTAGTTTCGAGGATAGTTTTACGTTACGA | SpeI |
| SacI-upA2 | GGCGCCGAGCTCCATATGACTCATCTTCAATCTTCCCCAAAT | SstI, NdeI |
| SacI-dnA2 | GCCGCCGAGCTCGGATCCTGAAGAACCTGAAAAATAGAAA | SstI, BamHI |
| NotI-dnA2 | GCCGCCGCGGCCGCTCCGAGACTCTTGCATACGA | NotI |
| AflII-upmtbA | CCGCCGCTTAAGAAAAATCTACTCCCAAACTA | AflII |
| NotI-upmtbA | CCGCCGGCGGCCGCACATGTTATAATCCCTCTCTAAGTTCTA | NotI, BspLU11I |
| NotI-dnmtbA | CCGCGGGCGGCCGCAGATCTGTAAACAAGTACTGAGTAATGC | NotI, BglII |
| KpnI-dnmtbA | CCGCCGGGTACCATTTATCAAAAATCTCTTGG | KpnI |
| A1L | GCACTATCTGCCCCCATTTA | None |
| A1R | AAGCTGCCCTTATGGGTTTT | None |
| A2L | TCGTTGCCCAAACAATGATA | None |
| A2R | GCAGTTCTTTCTCCCTGTGC | None |
| mtbA up | TAAATTGTGCACGGAACTGC | None |
| mtbA down | CTTCCGGAAAATTTGAGAGC | None |
| mtaA1for | GGCGCGCCTCCGTCCCTGAAGACTTTTG | AscI |
| mtaA1rev | TTTTTTACTTTTATCTTAGTACATATATTTTTATTGC | None |
| mtaA1rev2 | GGTCATACCTTTTTTACTTTTATCTTAGTACATATATTTTTATTG | None |
| mtaA2for | GGCGCGCCAATATCTGAAAGGACATTCTAAATCC | AscI |
| mtaA2rev | CAATCTTCCCCAAATATTCAATTCAC | None |
| mtbAfor | GGCGCGCCCACCATACCGCCCAGTATTT | AscI |
| mtbArev | TAATCCCTCTCTAAGTTCTATAGACAGC | None |
a
Added restriction sites are underlined.