TABLE 3.
M. acetivorans C2A strains used
| Straina | Genotype or description | Source or reference |
|---|---|---|
| C2A | Wild type (DSM 2834) | 37 |
| WWM1 | Δhpt | 33 |
| WWM13 | ΔmtaCB1 ΔmtaCB2 ΔmtaCB3 Δhpt | 32 |
| WWM21 | ΔmtaA1 Δhpt | This study |
| WWM27 | ΔmtaA2 Δhpt | This study |
| WWM28 | ΔmtaA2 ΔmtaCB1 ΔmtaCB2 ΔmtaCB3 Δhpt | This study |
| WWM33 | ΔmtaA1 ΔmtaA2 Δhpt | This study |
| WWM40 | ΔmtbA Δhpt | This study |
| WWM147 | ΔmtaA1 ΔmtaA2 ΔmtaCB1 ΔmtaCB2 ΔmtaCB3 Δhpt | This study |
| WWM346 | ΔmtaA1 ΔmtaCB1 ΔmtaCB2 ΔmtaCB3 Δhpt | This study |
| WWM82 | Δhpt::φC31 int-attP | 20 |
| WWM347 | Δhpt::φC31 int-attR PmtaA1-uidA (annotated start codon)-attL | This study |
| WWM348 | Δhpt::φC31 int-attR PmtaA1-uidA (alternate start codon)-attL | This study |
| WWM349 | Δhpt::φC31 int-attR PmtaA2-uidA (annotated start codon)-attL | This study |
| WWM350 | Δhpt::φC31 int-attR PmtbA-uidA-attL | This study |
a
All strains are derivatives of M. acetivorans WWM1 and were constructed by markerless exchange as described in Materials and Methods. Plasmids used for introduction of the ΔmtaA1, ΔmtaA2, and ΔmtbA alleles were pMP68, pMP70, and pMP65, respectively. The construction of WWM15 has been previously published (32) and was used to construct WWM28, WWM147, and WWM346.