TABLE 6.

Growth of ΔmtaA and mtbA mutants in various methanogenic substrates^a

Strain genotype	Substrate generation time (h)					Substrate lag time (h)					Substrate growth yield (maximum OD600)
	Methanol	TMA	DMA	MMA	Acetate	Methanol	TMA	DMA	MMA	Acetate	Methanol	TMA	DMA	MMA	Acetate
Δhpt	7.2 ± 0.2	6.5 ± 0.3	15.3 ± 0.6	11.7 ± 1.1	50.8 ± 2.9	29 ± 2	20 ± 1	44 ± 1	28 ± 1	124 ± 6	0.72 ± 0.01	0.82 ± 0.01	0.51 ± 0.03	0.39 ± 0.02	0.42 ± 0.1
ΔmtaA1 Δhpt	NG	8.5 ± 0.4	24.9 ± 3.0	12.8 ± 0.3	55.6 ± 0.8	NG	27 ± 2	53 ± 5	30 ± 2	159 ± 10	NG	0.85 ± 0.02	0.51 ± 0.02	0.38 ± 0.02	0.44 ± 0.1
ΔmtaA2 Δhpt	6.5 ± 0.6	8.8 ± 0.6	32.8 ± 0.9	21.6 ± 0.9	55.7 ± 3.1	18 ± 2	29 ± 1	68 ± 14	52 ± 3	138 ± 6	0.80 ± 0.04	0.83 ± 0.02	0.50 ± 0.02	0.39 ± 0.0	0.42 ± 0.0
ΔmtaA1 ΔmtaA2 Δhpt	NG	9.7 ± 0.9	28.5 ± 0.7	25.7 ± 0.6	57.1 ± 1.7	NG	34 ± 2	67 ± 2	56 ± 1	124 ± 2	NG	0.84 ± 0.04	0.51 ± 0.02	0.39 ± 0.01	0.44 ± 0.0
ΔmtaCB1 ΔmtaCB2 ΔmtaCB3 Δhpt	NG	8.7 ± 0.7	27.9 ± 0.3	30.2 ± 0.4b	72.7 ± 3.3	NG	25 ± 3	71 ± 3	134 ± 4b	241 ± 7	NG	0.85 ± 0.00	0.52 ± 0.01	0.36 ± 0.01b	0.4 ± 0.1
ΔmtaA1 ΔmtaCB1 ΔmtaCB2 ΔmtaCB3 Δhpt	NG	8.3 ± 0.1	25.1 ± 0.5	13.9 ± 0.4	NG	NG	33 ± 1	64 ± 2	31 ± 2	NG	NG	0.89 ± 0.01	0.46 ± 0.04	0.38 ± 0.02	NG
ΔmtaA2 ΔmtaCB1 ΔmtaCB2 ΔmtaCB3 Δhpt	NG	10.5 ± 0.9	26.6 ± 3.8	29.6 ± 0.6	85.2 ± 2.4	NG	40 ± 2	76 ± 2	21 ± 1	233 ± 18	NG	0.84 ± 0.01	0.51 ± 0.01	0.38 ± 0.02	0.4 ± 0.1
ΔmtaA1 ΔmtaA2 ΔmtaCB1 ΔmtaCB2 ΔmtaCB3 Δhpt	NG	8.4 ± 0.9	25.1 ± 0.5	17.3 ± 1.1	NG	NG	28 ± 3	64 ± 5	38 ± 2	NG	NG	0.84 ± 0.02	0.44 ± 0.02	0.36 ± 0.01	NG
ΔmtbA Δhpt	9.5 ± 1.4	21.2 ± 0.5	NG	NG	46.5 ± 1.1	29 ± 2	107 ± 5	NG	NG	109 ± 5	0.72 ± 0.01	0.42 ± 0.01	NG	NG	0.40 ± 0.02

^aGrowth was measured as indicated in Materials and Methods. Lag time represents the time required to reach one-half of the maximum OD value. Values represent the average and standard deviations of triplicate measurements. NG, no growth was seen for at least 3 months.

^bGrowth parameters for this strain on MMA were determined during a TMA-to-MMA substrate switch growth experiment.