TABLE 8.
β-Glucuronidase activities of uidA translational fusions to mtaA1, mtaA2 and mtbA in cells grown on various methanogenic substrates
| Fusion | Activity on the indicated substrate (mU/mg of protein)a
|
||||
|---|---|---|---|---|---|
| Methanol | TMA | DMA | MMA | Acetate | |
| mtaA1 (annotated) | 8 ± 0.3 | 6 ± 0.3 | 10 ± 1.8 | 7 ± 0.7 | 1.8 ± 0.5 |
| mtaA1 (alternate) | 157 ± 10 | 42 ± 3 | 65 ± 9 | 13 ± 3 | 68 ± 11 |
| mtaA2 | 8 ± 0.2 | 1 ± 0.1 | 2 ± 0.1 | 1 ± 0.5 | 2 ± 0.9 |
| mtbA | 418 ± 37 | 381 ± 36 | 460 ± 39 | 164 ± 38 | 22 ± 2 |
a
Activity was determined as nmol of β-glucuronidase produced min−1 mg−1 of protein Values represent the average and standard error of nine independent measurements. The limit of detection for this assay is 0.1 mU/mg of protein.