FIG. 2.

Northern blot analysis of RyeA and RyeB RNAs in Salmonella enterica serovar Typhimurium LT2. Cultures grown overnight in LB were diluted 1:200 in fresh LB and grown to an optical density at 600 nm of 0.35. RNA was extracted as previously described (4), fractionated on a 6% polyacrylamide-8 M urea gel (lane 1) or on an 8% polyacrylamide-8 M urea gel (lane 2), transferred onto a Hybond-N⁺ membrane, and hybridized to ³²P-labeled oligonucleotides complementary to RyeA (pp925 [5′-GGAAAACCTGGCGTCGTCATCTATTCTTAAAGGGCAAGGCGA-3′]) and RyeB (pB13 [5′-GATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAG-3′]). Sizes were estimated from migration distances of tmRNA and 5S RNA (not shown).