Relative NaCl sensitivities of various H. pylori strains. (A) H. pylori cultures of strains G27 and 26695 were grown in liquid BB5. The numbers of starting cells were normalized by using optical density at 600 nm. The relative salt sensitivity was assessed by serial dilution and plating on BB-based agar plates containing increasing concentrations of salt. CFU were enumerated, and the percentages of survival relative to the number of CFU on the BB5 plates are presented. (B) Relative growth of H. pylori strains G27 and 26695 on BB5 and BB15 plates. Serial dilutions of cultures normalized using optical density at 600 nm were plated side by side on BB5 and BB15. (C) H. pylori strains J99, J166, B128, SS1, HPAG1, K6, and K112 were examined as described above. An asterisk indicates that no single colonies were detected, and the bars below the asterisks indicate the limits of detection at the concentrations used. The results are representative of multiple biological repeats.