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Radio-TLC analyses of the products from the GGR reactions with GGGP (A) and GGPP (B). After hydrolysis with phosphatase, the compounds were analyzed by reversed-phase TLC developed with acetone/H2O (9:1). Lane 1, phosphatase-treated product from the reaction with S. acidocaldarius GGR; lane 2, phosphatase-treated product from the reaction with A. fulgidus GGR; lane 3, authentic geranylgeranylglycerol (A) and geranylgeraniol (B). s.f., solvent front; ori., origin.
